The field of cell culture has evolved significantly since its inception in the early 20th century when scientists successfully cultured cells outside of their natural environment. Many technological advancements and new methodologies have propelled the field forward since then and today, cell culture remains the cornerstone of biomedical research allowing researchers to study cellular mechanisms involved in biological development and disease.

Why Primary Cells Outshine Cell Lines

Primary cells, derived directly from living tissues, closely mimic the physiological state of cells in vivo. This makes them more representative of actual biological processes compared to immortalized cell lines, which often undergo genetic and phenotypic changes that diverge from the source tissue. Here are some key reasons why primary cells are preferred by researchers:

• Physiological Relevance: Primary cells maintain the specific characteristics of the tissue they originate from, providing a more accurate model for studying cellular functions and disease mechanisms. This is important for translational research and the development of therapeutics.
• Genetic Integrity: Unlike cell lines, which can undergo genetic drift and accumulate mutations over multiple passages, primary cells maintain their original genetic makeup, ensuring more consistent and reliable experimental outcomes.
• Cell Line Contamination: Many of the cell lines used in research today are misidentified or contaminated, which can result in compromised (or erroneous) data and wasted resources.

Lifeline’s Extensive Portfolio of Human Primary Cells

Lifeline® Cell Technology has established itself as a leader in providing high-quality primary cells to the global research community. Our extensive catalog includes a diverse array of human primary cell types including endothelial, epithelial, fibroblasts, hematopoietic, keratinocytes, melanocytes, mesenchymal stem cells, neural stem cells, skeletal muscle cells, and smooth muscle cells.

Each batch of cells undergoes rigorous quality testing to ensure they meet stringent quality control standards, so you know the cells you purchase will work in your research, every time. Lifeline has a wide variety of donors to choose from with different demographics (age, gender, ethnicity), which is especially useful in early drug testing to ensure the drug’s effectiveness across diverse populations.

Complete Cell Culture Systems

At Lifeline, we understand that successful cell culture depends on more than just the cells themselves. That’s why we offer complete media kits designed to meet the growth requirements for each cell type. Our media formulations do not contain antimicrobials or phenol red, components that can cause cell stress and “masking effects” that may negatively impact experimental results.

Whether your research focuses on oncology, immunology, neurology, or any other field, we have the cells you need. Our primary cells are trusted by researchers around the world and have been featured in numerous peer-reviewed publications—many of which we feature on our blog!

To learn more about Lifeline’s primary human cells, please visit: https://www.lifelinecelltech.com/product-category/cells/