Product Description

Human Peripheral Blood Mononuclear Cells are quality tested via flow cytometry to ensure proper expression of multiple markers of various hematopoietic lineage cells.

Lifeline® Peripheral Blood Mononuclear Cells are tested for:

• Cell Count:
≥ 25 x 10⁶ cells per vial
• Cell Viability:
Minimum 70% viability when thawed from cryopreservation
• Sterility Testing:
Negative for mycoplasma, bacterial and fungal growth
• Virus Testing:
Negative for HIV-1, HIV-2, HBV, and HCV by PCR
• Flow Cytometry Analysis:
Tested for CD3, CD4, CD8, CD16, CD19, CD56, CD235a

Normal Human Peripheral Blood Mononuclear Cells have not been exposed to antimicrobials or phenol red. Although antimicrobials are not required for eukaryotic cell proliferation, they are recommended to inhibit potential fungal or bacterial contamination of eukaryotic cell cultures.

Use RPMI1640-FBS Medium Complete Kit with Blood Mononuclear Cells. The Kit includes frozen basal medium containing phenol red, 2 mM L-glutamine, 25 mM HEPES and 1 mM sodium Pyruvate. The Complete Kit also includes Fetal Bovine Serum and GA (Gentamicin and Amphotericin B).