Normal Human Umbilical Vein Endothelial Cells (HUVEC), Primary

Product Description

Lifeline^® normal Primary Human Umbilical Vein Endothelial Cells (HUVEC), when grown in VascuLife^® Endothelial Culture Medium, provide an ideal low serum culture model, with or without human VEGF, for the study of angiogenesis, atherosclerosis or vascular biology.

• Lifeline Primary HUVEC may be cultured with or without VEGF.
• HUVEC can be grown in low serum (2%) medium without phenol red or antimicrobials when cultured in VascuLife medium.

Lifeline Primary HUVEC are isolated from human umbilical cords, and cryopreserved after primary culture to ensure the highest viability and plating efficiency. Lifeline HUVEC can come from a variety of donors without the accumulated mutations found in immortal cell lines.

• HUVEC are isolated in VascuLife EnGS Medium to ensure that they remain unexposed to recombinant human VEGF.
• Our HUVEC are quality tested in VascuLife 2% serum medium to ensure optimal reduced-serum growth over a period of at least 15 population doublings at rates equal to or greater than serum-supplemented medium.
• Lifeline Primary Umbilical Vein Endothelial Cells are not exposed to antimicrobials or phenol red when cultured in VascuLife medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results.

500,000 cells per vial

Lifeline umbilical vein endothelial cells have been quality tested for:

• Proliferation and Morphology:  Normal cell appearance for 15 population doublings
• Cell Viability:  Minimum 70% viability when thawed from cryopreservation
• Sterility:  Negative for mycoplasma. Negative for bacterial and fungal growth
• Virus:  Negative for HIV-1, HIV-2, HBV, and HCV by PCR
• Specific Staining:  von Willebrand Factor positive. Smooth muscle a-actin negative.