Normal Human Primary Lung Fibroblasts

Product Description

Lifeline® normal Human Primary Lung Fibroblasts are directly cultured from their source organ tissue, to help ensure valid research outcomes.  Primary Cells come from a wide variety of donors without the accumulated mutations found in immortal cell lines.

Primary Lung Fibroblasts provide an ideal cell system to:

• Study wound healing, toxicology, cancer, or basic cell biology.
• Use for drug screening, drug development, and genome editing applications.
• Establish serum free human feeder layers for human embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), and other cell culture applications requiring feeder layers.

Primary Lung Fibroblasts have been isolated, plated and expanded in culture vessels once before being harvested for cryopreservation to ensure the highest purity, viability and plating efficiency.

Our Lung Fibroblasts are quality tested in FibroLife S2 Low Serum Fibroblast Medium  or FibroLife Xeno-Free Fibroblast Frozen Medium and demonstrate optimal growth at rates equal to or greater than classical serum-supplemented media.  Fibroblasts are tested for:

• Proliferation and morphology:  normal cell appearance for a period of at least 15 population doublings.
• Cell Viability:  Minimum 70% viability when thawed from cryopreservation
• Sterility Testing:  Negative for mycoplasma. Negative for bacterial and fungal growth.
• Virus Testing:  Negative for HIV-1, HIV-2, HBV, and HCV by PCR

Lifeline Primary Lung Fibroblasts need not be exposed to antimicrobials or phenol red when cultured in FibroLife Medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results. FibroLife Medium has been composed and supplemented specifically to maximize the life expectancy of Primary Fibroblasts.

500,000 cells per vial