Human Cell Types and Their Research Applications

Lifeline® Normal Human Cells have been validated for numerous applications that cover diverse research interests . . .

Cancer research. Many Lifeline® normal human cell types are used as normal controls in cancer studies to demonstrate specificity of drugs for cancer cells. In these studies, cancer drugs largely do not affect normal cells.

Lung injury and disease. Many Lifeline® airway cell types, including epithelial cells, fibroblasts, and smooth muscle cells have been used in customer research to study mechanisms of influenza infection, as well as lung cancer therapies, and more.

Gingivitis and oral health. Lifeline® Oral cell types, including oral keratinocytes and gingival fibroblasts, can be used to study oral health, including conditions like gingivitis.

Bacterial and fungal infection. In particular, Lifeline® keratinocytes have been used to study the pathogenesis and drug resistant properties of the bacteria Acinetobacter baumannii.

Skin conditions and diseases. Given the upcoming summer months and the potential exposure to UV radiation, the risk of skin cancer will be on everyone’s minds. Lifeline® dermal fibroblasts and normal melanocytes can be used to study the effects of UV radiation, as well as potential therapies for melanoma.

Stem cell and regenerative medicine. Since the first report in 2007, much attention has been given to induced pluripotent stem cells (iPS cells) and their potential for regenerative medicine in the clinic. Importantly, Lifeline® dermal fibroblasts have been successfully reprogrammed into iPS cells and subsequently differentiated into motor neurons.

Cardiovascular disease and therapies. Cardiovascular cells, including endothelial cells and smooth muscle cells, have been used to study atherosclerosis, angiogenesis, and thrombosis.

Wound healing and fibrotic diseases. One of the major cell types involved in wound healing throughout the body is the fibroblast. Lifeline® offers fibroblasts from the mouth, bladder, skin, lung, uterus, and vas deferens.

DNA-based therapeutic development. Dermal fibroblasts have also been used as normal control cells for transfection of antisense oligonucleotides against STAT3. Antisense oligonucleotides are being evaluated as a therapeutic option to neutralize molecules, such as STAT3, that become dysregulated in cancer.

Medical device diagnostic development. Smooth muscle cells have been used to engineer neovessels in vitro, with the hope of improving the success of tissue-engineered vascular grafts. Additionally, Lifeline® endothelial and smooth muscle cells have been used to develop MRI imaging techniques to enable imaging of two cell populations simultaneously.

Antiviral and immune responses. Airway epithelial cells provide an excellent model for viral infection and have been used to study the effects of influenza. Additionally, Lifeline® HUVECs have been used as a model to study neutrophil transmigration through endothelial cells during an immune response.

To read more about these studies and more, please visit our references page at: http://www.Lifelinecelltech.com/technical-support/references/

Lifeline® provides primary and secondary cells from both human and mouse, suitable for your research needs. Lifeline® human cell types are isolated from:

• Adipose
• Bladder
• Hematopoietic system
• Bone marrow
• Breast
• Cardiovascular system
• Cornea
• Kidney
• Lung
• Mouth
• Prostate
• Reproductive system
• Skin
• Umbilical cord

These include epithelial cells, endothelial cells, fibroblasts, smooth muscle cells, and stem cells. In addition, mouse dendritic cells are available.