FibroLife S2 Fibroblast Medium Complete Kit

Product Description

Optimized for Culturing Primary Human Fibroblasts and Pre-Adipocyte Mesenchymal Stem Cells in a Low-Serum Environment.  FibroLife S2 supports the growth of these cells in a 2% serum environment at rates equal to or greater than comparable media supplemented with 2% to 10% FBS.

Primary Cells cultured in FibroLife medium out-perform cells grown in any similar medium

You’ll get consistently reproducible results with FibroLife medium. FibroLife is balanced and supplemented expressly for Primary Human Fibroblasts to maximize that individual cell type’s life expectancy.

• Your Primary Fibroblasts will plate better using FibroLife optimized media (as compared to our nearest competitors’ media or traditional high-serum media)
• Your Primary Fibroblasts will grow more quickly in FibroLife medium than in other commercially-available media
• Primary Fibroblasts will maintain better morphology when cultured in FibroLife medium

FibroLife Basal Medium contains no antimicrobials and no phenol red. Antimicrobials and phenol red are not required for eukaryotic cell proliferation, but are available from Lifeline.

FibroLife S2 provides an ideal cell system to establish low-serum human feeder layers for stem cell culture or as a model to study wound healing, toxicology or basic cell biology.

Kit format for formulation flexibility:  Offered in a kit format comprised of FibroLife Basal Medium in a light-protected 500 mL bottle, and FibroLife S2 LifeFactors Kit with associated supplements and growth factors. This kit allows you to prepare fresh medium in your laboratory, extending shelf life and enhancing performance.

ISO-Style Quality Testing for Reproducible Results

FibroLife S2 medium is tested for:

• Sterility: Negative for bacteria and fungal growth
• pH: 7.4 +/- 0.2
• Cell Performance:  Rate of proliferation and morphology
• Osmolality:  270 +/- 10 mOsm
• Endotoxin:  < 0.5 EU/mL

Superior proliferation: FibroLife S2 medium grows Primary Fibroblasts and pre-adipocytes in low-serum conditions at rates that meet or exceed other commercially available serum-containing media while maintaining excellent cell morphology. In comparisons with other commercially-available media, FibroLife S2 medium shows superior proliferation at different seeding densities.

Shown at left: Primary Human Dermal Fibroblasts-Neonatal, passage 2, 5 days after inoculation with 2,000 cells/cm² (100X)

Shown below: Primary Human Dermal Fibroblasts-Neonatal inoculated at low seeding densities in 24-well plates. Higher fluorescence represents a greater number of cells per well, and therefore, better proliferation.